Fig. 5. Combination of 7-KC and TRIOL induces independent activation of RBC-NOX and RBC-NOS. Measurements of DAF-FM or DCF associated cell fluorescence ( grey and white columns, respectively) and PS externalization (black columns) were performed after a 2 h and 24h incubation, respectively, in RBCs treated with TRIOL (2 µM) or 7-KC (7 µM) or both preceded by 1 h pre-treatment in the absence or in the presence of DPI or L-NAME or both. Values are the mean ±SD of three separate experiments carried out in duplicate. Within homologue determinations, values with different letters are significantly different (P<0.0001) (one-way Anova associated with Bonferroni's post test).